Development of sequence characterized amplified region (SCAR) primers for the detection of Phyto.5.2, a major QTL for resistance to Phytophthora capsici Leon. in pepper.
Identifieur interne : 002207 ( Main/Exploration ); précédent : 002206; suivant : 002208Development of sequence characterized amplified region (SCAR) primers for the detection of Phyto.5.2, a major QTL for resistance to Phytophthora capsici Leon. in pepper.
Auteurs : E A Quirin [États-Unis] ; E A Ogundiwin ; J P Prince ; M. Mazourek ; M O Briggs ; T S Chlanda ; K-T Kim ; M. Falise ; B-C Kang ; M M JahnSource :
- TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik [ 0040-5752 ] ; 2005.
Descripteurs français
- KwdFr :
- Amorces ADN (MeSH), Capsicum (génétique), Capsicum (microbiologie), Cartographie chromosomique (MeSH), Chromosomes de plante (MeSH), Clonage moléculaire (MeSH), Gènes de plante (MeSH), Locus de caractère quantitatif (MeSH), Maladies des plantes (MeSH), Marqueurs génétiques (MeSH), Phytophthora (physiologie), Technique RAPD (MeSH).
- MESH :
- génétique : Capsicum.
- microbiologie : Capsicum.
- physiologie : Phytophthora.
- Amorces ADN, Cartographie chromosomique, Chromosomes de plante, Clonage moléculaire, Gènes de plante, Locus de caractère quantitatif, Maladies des plantes, Marqueurs génétiques, Technique RAPD.
English descriptors
- KwdEn :
- Capsicum (genetics), Capsicum (microbiology), Chromosome Mapping (MeSH), Chromosomes, Plant (MeSH), Cloning, Molecular (MeSH), DNA Primers (MeSH), Genes, Plant (MeSH), Genetic Markers (MeSH), Phytophthora (physiology), Plant Diseases (MeSH), Quantitative Trait Loci (MeSH), Random Amplified Polymorphic DNA Technique (MeSH).
- MESH :
- chemical : DNA Primers, Genetic Markers.
- genetics : Capsicum.
- microbiology : Capsicum.
- physiology : Phytophthora.
- Chromosome Mapping, Chromosomes, Plant, Cloning, Molecular, Genes, Plant, Plant Diseases, Quantitative Trait Loci, Random Amplified Polymorphic DNA Technique.
Abstract
Phytophthora capsici causes devastating disease on many crop species, including Capsicum. Resistance in Capsicum annuum is genetically and physiologically complex. A panel of Capsicum germplasm that included genotypes from both C. annuum and C. chinense showing highly resistant, highly susceptible and intermediate or tolerant responses to the pathogen, respectively, was screened with a series of randomly amplified polymorphic sequence primers to determine which genomic regions contribute to the highest level of resistance. One primer, OpD04, amplified a single band only in those C. annuum and C. chinense genotypes showing the highest level of resistance. The amplified product was cloned, sequenced and used to design longer primers in order to generate a sequence characterized amplified region marker which was then mapped in a reference mapping population and a screened population segregating for resistance to P. capsici. These primers were observed to define a locus on pepper chromosome 5 tightly linked to Phyto.5.2, one of six quantitative trait loci (QTL) previously reported to contribute to P. capsici resistance. These results indicate that the Phyto.5.2 QTL may be widely distributed in highly resistant germplasm and provide improved resolution for this QTL. This work also defines the first breeding tools for this system, allowing for the rapid selection of genotypes likely to be highly resistant to P. capsici.
DOI: 10.1007/s00122-004-1874-7
PubMed: 15657741
Affiliations:
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Mazourek</name></author><author><name sortKey="Briggs, M O" sort="Briggs, M O" uniqKey="Briggs M" first="M O" last="Briggs">M O Briggs</name></author><author><name sortKey="Chlanda, T S" sort="Chlanda, T S" uniqKey="Chlanda T" first="T S" last="Chlanda">T S Chlanda</name></author><author><name sortKey="Kim, K T" sort="Kim, K T" uniqKey="Kim K" first="K-T" last="Kim">K-T Kim</name></author><author><name sortKey="Falise, M" sort="Falise, M" uniqKey="Falise M" first="M" last="Falise">M. Falise</name></author><author><name sortKey="Kang, B C" sort="Kang, B C" uniqKey="Kang B" first="B-C" last="Kang">B-C Kang</name></author><author><name sortKey="Jahn, M M" sort="Jahn, M M" uniqKey="Jahn M" first="M M" last="Jahn">M M Jahn</name></author></analytic><series><title level="j">TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik</title><idno type="ISSN">0040-5752</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Capsicum (genetics)</term><term>Capsicum (microbiology)</term><term>Chromosome Mapping (MeSH)</term><term>Chromosomes, Plant (MeSH)</term><term>Cloning, Molecular (MeSH)</term><term>DNA Primers (MeSH)</term><term>Genes, Plant (MeSH)</term><term>Genetic Markers (MeSH)</term><term>Phytophthora (physiology)</term><term>Plant Diseases (MeSH)</term><term>Quantitative Trait Loci (MeSH)</term><term>Random Amplified Polymorphic DNA Technique (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Amorces ADN (MeSH)</term><term>Capsicum (génétique)</term><term>Capsicum (microbiologie)</term><term>Cartographie chromosomique (MeSH)</term><term>Chromosomes de plante (MeSH)</term><term>Clonage moléculaire (MeSH)</term><term>Gènes de plante (MeSH)</term><term>Locus de caractère quantitatif (MeSH)</term><term>Maladies des plantes (MeSH)</term><term>Marqueurs génétiques (MeSH)</term><term>Phytophthora (physiologie)</term><term>Technique RAPD (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>DNA Primers</term><term>Genetic Markers</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Capsicum</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Capsicum</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Capsicum</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Capsicum</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Phytophthora</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Chromosome Mapping</term><term>Chromosomes, Plant</term><term>Cloning, Molecular</term><term>Genes, Plant</term><term>Plant Diseases</term><term>Quantitative Trait Loci</term><term>Random Amplified Polymorphic DNA Technique</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Amorces ADN</term><term>Cartographie chromosomique</term><term>Chromosomes de plante</term><term>Clonage moléculaire</term><term>Gènes de plante</term><term>Locus de caractère quantitatif</term><term>Maladies des plantes</term><term>Marqueurs génétiques</term><term>Technique RAPD</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Phytophthora capsici causes devastating disease on many crop species, including Capsicum. Resistance in Capsicum annuum is genetically and physiologically complex. A panel of Capsicum germplasm that included genotypes from both C. annuum and C. chinense showing highly resistant, highly susceptible and intermediate or tolerant responses to the pathogen, respectively, was screened with a series of randomly amplified polymorphic sequence primers to determine which genomic regions contribute to the highest level of resistance. One primer, OpD04, amplified a single band only in those C. annuum and C. chinense genotypes showing the highest level of resistance. The amplified product was cloned, sequenced and used to design longer primers in order to generate a sequence characterized amplified region marker which was then mapped in a reference mapping population and a screened population segregating for resistance to P. capsici. These primers were observed to define a locus on pepper chromosome 5 tightly linked to Phyto.5.2, one of six quantitative trait loci (QTL) previously reported to contribute to P. capsici resistance. These results indicate that the Phyto.5.2 QTL may be widely distributed in highly resistant germplasm and provide improved resolution for this QTL. This work also defines the first breeding tools for this system, allowing for the rapid selection of genotypes likely to be highly resistant to P. capsici.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15657741</PMID><DateCompleted><Year>2005</Year><Month>05</Month><Day>31</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>10</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0040-5752</ISSN><JournalIssue CitedMedium="Print"><Volume>110</Volume><Issue>4</Issue><PubDate><Year>2005</Year><Month>Feb</Month></PubDate></JournalIssue><Title>TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik</Title><ISOAbbreviation>Theor Appl Genet</ISOAbbreviation></Journal><ArticleTitle>Development of sequence characterized amplified region (SCAR) primers for the detection of Phyto.5.2, a major QTL for resistance to Phytophthora capsici Leon. in pepper.</ArticleTitle><Pagination><MedlinePgn>605-12</MedlinePgn></Pagination><Abstract><AbstractText>Phytophthora capsici causes devastating disease on many crop species, including Capsicum. Resistance in Capsicum annuum is genetically and physiologically complex. A panel of Capsicum germplasm that included genotypes from both C. annuum and C. chinense showing highly resistant, highly susceptible and intermediate or tolerant responses to the pathogen, respectively, was screened with a series of randomly amplified polymorphic sequence primers to determine which genomic regions contribute to the highest level of resistance. One primer, OpD04, amplified a single band only in those C. annuum and C. chinense genotypes showing the highest level of resistance. The amplified product was cloned, sequenced and used to design longer primers in order to generate a sequence characterized amplified region marker which was then mapped in a reference mapping population and a screened population segregating for resistance to P. capsici. These primers were observed to define a locus on pepper chromosome 5 tightly linked to Phyto.5.2, one of six quantitative trait loci (QTL) previously reported to contribute to P. capsici resistance. These results indicate that the Phyto.5.2 QTL may be widely distributed in highly resistant germplasm and provide improved resolution for this QTL. This work also defines the first breeding tools for this system, allowing for the rapid selection of genotypes likely to be highly resistant to P. capsici.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Quirin</LastName><ForeName>E A</ForeName><Initials>EA</Initials><AffiliationInfo><Affiliation>Department of Plant Breeding and Genetics, Cornell University, Ithaca, NY, I4853, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ogundiwin</LastName><ForeName>E A</ForeName><Initials>EA</Initials></Author><Author ValidYN="Y"><LastName>Prince</LastName><ForeName>J P</ForeName><Initials>JP</Initials></Author><Author ValidYN="Y"><LastName>Mazourek</LastName><ForeName>M</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Briggs</LastName><ForeName>M O</ForeName><Initials>MO</Initials></Author><Author ValidYN="Y"><LastName>Chlanda</LastName><ForeName>T S</ForeName><Initials>TS</Initials></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>K-T</ForeName><Initials>KT</Initials></Author><Author ValidYN="Y"><LastName>Falise</LastName><ForeName>M</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Kang</LastName><ForeName>B-C</ForeName><Initials>BC</Initials></Author><Author ValidYN="Y"><LastName>Jahn</LastName><ForeName>M M</ForeName><Initials>MM</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D023362">Evaluation Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2005</Year><Month>01</Month><Day>19</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Theor Appl Genet</MedlineTA><NlmUniqueID>0145600</NlmUniqueID><ISSNLinking>0040-5752</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017931">DNA Primers</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005819">Genetic Markers</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002212" MajorTopicYN="N">Capsicum</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002874" MajorTopicYN="N">Chromosome Mapping</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032461" MajorTopicYN="N">Chromosomes, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017931" MajorTopicYN="Y">DNA Primers</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017343" MajorTopicYN="Y">Genes, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005819" MajorTopicYN="N">Genetic Markers</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010838" MajorTopicYN="N">Phytophthora</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D040641" MajorTopicYN="Y">Quantitative Trait Loci</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019105" MajorTopicYN="N">Random Amplified Polymorphic DNA Technique</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2004</Year><Month>09</Month><Day>16</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2004</Year><Month>11</Month><Day>06</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2005</Year><Month>1</Month><Day>20</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2005</Year><Month>6</Month><Day>1</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2005</Year><Month>1</Month><Day>20</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">15657741</ArticleId><ArticleId IdType="doi">10.1007/s00122-004-1874-7</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Genetics. 1997 Nov;147(3):1469-85</Citation><ArticleIdList><ArticleId IdType="pubmed">9383086</ArticleId></ArticleIdList></Reference><Reference><Citation>Genomics. 1987 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IdType="pubmed">12750791</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>État de New York</li></region><settlement><li>Ithaca (New York)</li></settlement><orgName><li>Université Cornell</li></orgName></list><tree><noCountry><name sortKey="Briggs, M O" sort="Briggs, M O" uniqKey="Briggs M" first="M O" last="Briggs">M O Briggs</name><name sortKey="Chlanda, T S" sort="Chlanda, T S" uniqKey="Chlanda T" first="T S" last="Chlanda">T S Chlanda</name><name sortKey="Falise, M" sort="Falise, M" uniqKey="Falise M" first="M" last="Falise">M. Falise</name><name sortKey="Jahn, M M" sort="Jahn, M M" uniqKey="Jahn M" first="M M" last="Jahn">M M Jahn</name><name sortKey="Kang, B C" sort="Kang, B C" uniqKey="Kang B" first="B-C" last="Kang">B-C Kang</name><name sortKey="Kim, K T" sort="Kim, K T" uniqKey="Kim K" first="K-T" last="Kim">K-T Kim</name><name sortKey="Mazourek, M" sort="Mazourek, M" uniqKey="Mazourek M" first="M" last="Mazourek">M. Mazourek</name><name sortKey="Ogundiwin, E A" sort="Ogundiwin, E A" uniqKey="Ogundiwin E" first="E A" last="Ogundiwin">E A Ogundiwin</name><name sortKey="Prince, J P" sort="Prince, J P" uniqKey="Prince J" first="J P" last="Prince">J P Prince</name></noCountry><country name="États-Unis"><region name="État de New York"><name sortKey="Quirin, E A" sort="Quirin, E A" uniqKey="Quirin E" first="E A" last="Quirin">E A Quirin</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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